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KMID : 0608520000060010089
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Korean Journal of Oriental Medicine
2000 Volume.6 No. 1 p.89 ~ p.98
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Large Scale Gene Expression Analysis in Rat Models of 4-Vessel Occlusion Ischemia
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강봉주
홍성길/김윤택/김영옥/조동욱
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Abstract
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Cerebral ischemia, the most prevalent form of clinical stroke, is a medical problem of the first magnitude. Substantial efforts are being made to develop drugs which will protect the brain from the neurodegeneration followed by an ishemic stroke. A key factor in this process is the increasing an evidence that free radical is involved in the mechanisms of ischemic brain damage. We investigated the macro scale gene expression analysis on the global ischemic induced by 4-vessel occlusion in Wister rats. The recent availability of microarrays provides an attractive strategy for elaborating an unbiased molecular profile of large number of genes during ischemic injury. This experimental approach offers the potential to identify molecules or cellular pathways not previously associated with ischemia. ischemia was induced by 4-vessel occlusion for 10 minutes and reperfused again. RNA from sham control brain and time-dependent ischemed brain were hybridized to microarrays containing 4,000 rat genes. 589 genes were found to be at least 2 fold regulated at one or more time points. These survey data provide the foundation studies that should provide convincing proof for ischemia and oxidative stress on gene expression.
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KEYWORD
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Ischemia , 4-vessel occlusion , DNA chip , gene expression
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